Sterile bottle top vacuum filtration is a method used to filter and sterilize liquid samples. It involves using a vacuum pump to apply negative pressure to a filter unit with a membrane filter on top, which is then attached to a bottle or flask containing the liquid sample. The negative pressure forces the liquid through the filter membrane, removing any particulate matter or microorganisms that may be present in the sample.

This technique is commonly used in microbiology and molecular biology applications, where a sterile environment is crucial to prevent contamination and ensure accurate results. It is especially useful for sterilizing large volumes of liquid samples, such as media, buffers, and culture supernatants.

To perform sterile bottle top vacuum filtration, the filter unit and membrane filter must first be sterilized using methods such as autoclaving or gamma irradiation. The liquid sample is then poured into the sterile bottle or flask, and the filter unit is attached to the top. The vacuum pump is turned on, and the negative pressure forces the liquid through the filter membrane into the sterile bottle or flask below.

After filtration, the filter unit can be detached and discarded, while the filtered liquid can be used for further experiments or analysis. Sterile bottle top vacuum filtration is a simple, efficient, and cost-effective method for sterilizing liquid samples and is widely used in various scientific applications.

Application:
Ideal for filtration of tissue culture media, biological fluids, fixation buffers etc
Cell culture media and other aqueous solutions
Sterile filtration of solutions which can’t be auto-claved
Sterile filtration and clarification of difficult-to-filter
aqueous solutions with a glass fiber pre-filter


Quality assuranceQuality assurance
1. ISO9000 certified manufacturing:highest standards, certified clean room conditions
2. All the syringe filters are certified by HPLC Extractable Test
3. Highly asymmetric PES membrane:high flow rate and throughput
4. 100% integrity testing