Onechanbara: Bikini Samurai Squad is the first game for the Oneechanbara franchise for the Xbox 360. As it does for all Oneechanbara games, this title features sisters Aya and Saki, dressed in their respective trademark bikini/cowboy hat and schoolgirls' uniform, in their fight to eradicate a looming zombie infestation with samurai sword in hand and a stern expression in their eyes. Along for the fight is Annna, new to the franchise with her use of guns only for combat; also returning as an NPC is the motorcycle jumpsuit-wearing Reiko, who offers a balance between swordplay and gunplay.

The Oneechanbara franchise is in the beat em' up genre and similar in style to the Dynasty Warriors and Devil May Cry series. Familiar gameplay elements returning include: the emphasis on correct timed attacks (cool combos), sword fatigue that affects the timing and effectiveness of your characters attacks, playing two heroines concurrently which enables tagging out to regain lost health, and a rage meter that transforms the protagonist into a devil state when full, enabling both double damage exacted to enemy and oneself.