A portable version of it's console cousins, Def Jam Fight for NY: The Takeover features the same kind of gameplay, a fighting game using some of rap's biggest names and authentic music. The story mode leads up to Def Jam Vendetta, telling the story of how D-Mob takes control of New York. It also has new locations along with new moves as well.

Players can take control of rappers like Busta Rhymes, Carmen Electra, Lil' Kim, Ludacris, Method Man, Redman, Snoop Dogg, just to name a few, each with their own music provided to the game. Players can also create their own fighters using clothing from authentic designers like Ecko Unlimited, Sean John, and Reebok along with many others. Players can also go head-to-head using wireless.